Table 1 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana

Table 1 Effect of sucrose and growth regulators on friable embryogenic callus (FEC) production for somatic embryo (SE) development with 6 weeks of incubation in cell suspension culture of L. montana.
Sucrose (g L − 1) + PGR (μM) in solid MS mediumNumber of SEs/SCV developmental stageGermination (%)6 weeks of cultureGlobularDifferent stages of embryo*Control00030 + 10 2,4-D4.0 ef3.0 gh035 + 10 2,4-D6.2 de4.6 ef040 + 10 2,4-D7.8 cd6.4 de035 + 10 2,4-D + 2 BA9.4 c8.0 c035 + 10 2,4-D + 2 TDZ14.8 ab9.8 a030 + 10 picloram3.6 f2.0 h035 + 10 picloram7.2 cd5.0 ef040 + 10 picloram10.4 c6.6 de035 + 10 picloram + 2 BA16.2 a9.2 ab035 + 10 picloram + 2 TDZ9.8 c7.0 cd0
FEC = Friable embryogenic callus. PGR = Plant growth regulator.SEs = Somatic embryos. SCV = Settled cell volume. *Different stages of embryo = pear-shaped, early torpedoshaped, torpedo-shaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate. Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.