Data from: Multiplexed microsatellite markers for genetic studies of beech

European beech (Fagus sylvatica L.) is one of the economically most
important broadleaved tree species in Europe and has become a model for
studying climate change effects. Multiplex PCR of microsatellites is a
fast and cost-effective technique allowing high throughput genotyping.
Here we present the procedure used to develop two multiplex kits
(8-plexes) for European beech. We paid particular attention to quality
control throughout all steps of the multiplex kits development (null
allele detection, error rate measurements, linkage disequilibrium).
Preliminary assays suggest that the multiplex kits amplify 16 loci largely
devoid of null alleles and allow rapid and cost-effective genotyping of
beech with low error rates. The two kits, which differ in their levels of
polymorphism, most likely due to marker origin, were also informative in
seven other beech species tested.