Effect of efferent duct ligatation on the transcriptome of the first 3 proximal segments of the murine epididymis

To examine the effect of efferent duct ligation (EDL) on the gene expression of epididymal segments 1-3, we performed EDL procedures on WT mice and harvested the epididymides 3-weeks after surgery and sequenced the transcriptome of individual segments 1, 2, and 3 of ligated epididymides and control (non-ligated) epididymides Overall design: Comparative gene expression profiling analysis of RNA-seq data from segments 1, 2 and 3 of ligated epididymides (L1, L2, L3) after efferent duct ligations (EDL) and segments 1-3 of control (non-ligated) epididymides (C1, C2, C3). Two batches of sequencing runs were performed (3017 and 3097) each containing one of each sample (L1, L2, L3, C1, C2, C3)