Transcriptional signatures of heroin intake and relapse throughout the brain reward circuitry in male mice. Transcriptional signatures of heroin intake and relapse throughout the brain reward circuitry in male mice

Identifying changes in gene expression throughout the brain reward system induced across various conditions of heroin self-administration in mice as a model of opioid use disorder Overall design: Comparative gene expression profiling using bulk RNA-seq from brain tissue following experimental procedures: S24: 15d saline intravenous self-administration, 24h withdrawal H24: 15d heroin intravenous self-administration, 24h withdrawal SS: 15d saline intravenous self-administration, 30d withdrawal, saline challenge + 2h drug-seeking test SH: 15d saline intravenous self-administration, 30d withdrawal, heroin challenge + 2h drug-seeking test HS: 15d heroin intravenous self-administration, 30d withdrawal, saline challenge + 2h drug-seeking test HH: 15d heroin intravenous self-administration, 30d withdrawal, heroin challenge + 2h drug-seeking test