DD Neuron Outgrowth and Stability.
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The axon morphology of the six dorsal D (DD) motor neurons was visualized with Pflp-13GFP (juIs145) and Punc-25GFP (juIs76) transgenes in L1 larvae and with Pflp-13GFP in young adults (see Methods). All 6 DD axons were evaluated in animals expressing juIs76, and DD1-DD5 axons were examined in worms that expressed the juIs145 reporter. Animals were scored for gaps along the dorsal nerve cord, commissures that stopped short of the dorsal nerve cord, and abnormal branching morphology where commissures join the dorsal nerve cord (Figure 3). tbb-2(gk130) mutants and tba-1(ju89);tbb-2(gk130) animals exhibit similar DD axon defects, whereas axon defects are increased in tba-1(ju89);tbb-1(gk207) double mutants.*Two-tailed Z-test, p**p
创建时间：
2015-12-02
