Evolution of advanced chronic lymphoid leukaemia unveiled by single-cell transcriptomics: a case study. Evolution of advanced chronic lymphoid leukaemia unveiled by single-cell transcriptomics: a case study

Characterization of the transcriptional changes occurring at the single-cell level during the progression of a chronic lymphoid leukaemia treated with Rituximab, Fludarabine and Cyclophosphamide. Overall design: Single-cell RNA-seq library from peripheral blood cells of a subject with chronic lymphoid leukaemia; samples at diagnosis and relapse were separatly barcoded and pooled; single-cell captured using 10x Chromium and subjected to high-throughput sequencing.