Chromatin Occupancy of AR, BRD4, and H3K27Ac in MR42D Cells in response to Enzalutamide [ATAC-seq]

In order to look at global chromatin accessibility in enzalutamide sensitive (V16D) and enzalutamide-resistant t-NEPC lines (MR42D), we treated these lines with enzalutamide or vehicle and performed an Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-seq). Hyperaccessible regions in V16D were associated with metabolic pathways, whereas hyperaccesible regions in MR42D were associated with neuronal pathways, indicating that the chromatin conformation in these enzalutamide resistant cells is conducive to transdifferentiation to a neuronal t-NEPC phenotype. MR42D cells were grown in the presence of enzalutamide (Maintenance_MDV conditon) for 96 hours, or in the absence of enzalutamide for 72 hours, then enzalutamide (Washout_MDV) or Vehicle (Washout_Veh) was added back to the media and cells were cultured an additional 24 hours. Biological duplicates of the MR42D treatments were subjected to Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-seq). V16D cells were treated with enzalutamide or vehicle for 24 hours, and biological triplicates for each treatment were subjected to ATAC-seq.