Bacillus siamensis CML548 Genome sequencing and assembly. Bacillus siamensis strain:CML548

In our previous study, a total of 52 strains of Bacillus were isolated from the cecum of chickens from 14 breeds. After comparison of the biological characteristics of these isolates, a Bacillus siamensis strain named CML548 was selected for analysis in this study. In vitro experiment suggested that this strain possessed good probiotic features: having the ability to inhibit the growth of Escherichia, Salmonella Typhimurium, and Clostridium perfringens; highly tolerant to acid and bile salts; and producing protease, amylase and cellulase. To explore the underlying probiotic mechanisms of B. siamensis CML548, whole-genome analysis was performed. The results showed that the genome size of B. siamensis CML548 was 4 061 741 bp including a total of 3 961 coding genes with a GC content of 46.07%. The genome was annotated by using the GO, KEGG, COG, TCDB, CAZy, DBAASP, CARD and VFDB databases. Further, multiple secondary metabolic gene clusters involved in the synthesis of antibacterial active substances were predicted by using antiSMASH. Additionally, CML548 contained an antibacterial protein encoding gene tasA. In conclusion, B. siamensis CML548 isolated in the present study is a candidate probiotic and has the potential to be used as a feed additive.