Peptidomic and proteomic analysis of precision-cut lung slice supernatants

The data provided here is related to the publication “Peptidomic and proteomic analysis of precision-cut lung slice supernatants”. In this study, we sought out to develop a method that could incorporate a peptidomic and proteomic endpoint within the same workflow. The developed workflow was adapted from single-pot, solid-phase-enhanced sample preparation (SP3) for examining the proteome and peptidome of precision-cut lung slice supernatants in one experimental procedure to save sample material. A part of the study compared potential differences when utilizing different organic solvents for binding peptides and proteins to magnetic beads for biological replicates of PCLS supernatant. In connection, the workflow robustness was also characterized by investigating technical replicates of the same biological PCLS supernatant sample.