Differential expression of miRNAs in EGF treated HTR-8/SVneo versus untreated control by next generation sequencing

The goal of this study is to compare miRNAs expressed by EGF treated HTR-8/SVneo cells to miRNAs expressed in untreated control HTR-8/SVneo cells to identify micro RNAs which play a role during EGF-mediated HTR-8/SVneo cell invasion cDNA libraries were made from total RNA of untreated control and 24 h EGF treated samples by TruSeq Small RNA Sample prep kit. Deep sequencing of cDNA libraries were performed with the help of Illumina Genome analyzer IIx. Raw sequence data was imported into the CLC Genomics Workbench 6.5.1. The sequence reads were trimmed for adapter sequences and low quality base. The trimmed raw sequences were subjected to miRNA-sequence analysis, by mapping them to miRBase release 21.