Sequencing success rate associated with different molecular markers from pearl DNA extracts of Pinctada margaritifera, P. maxima and P. radiata using methods A, B and C (Fig. 2).

ain methods A and B the pearls were broken open using forceps to expose the inner material used to extract DNA. In method C the powder used for DNA extraction was obtained by drilling a 1-mm diameter hole in the pearls and the hole was enlarged internally using a 0.9 mm drill head.bpercentage (%) of successfully identified pearls (identified pearls/total pearls tested).cfrom a total of twelve P. maxima and P. margaritifera samples analyzed in method A or in method B, one pearl that was predicted to belong to P. maxima based morphological criteria was identified as P. margaritifera according to the DNA fingerprint.