Real-time quantitative PCR analysis of EV-containing miRNA from human adipocytes SGBS cells. Real-time quantitative PCR analysis of EV-containing miRNA from human adipocytes SGBS cells

Mature adipocytes were pretreated with VD for 24h and/or treated with TNFα during 24h. EVs released by adipocytes after TNF treatment were isolated and the miRNAs contained in it were extracted for qPCR analysis. Overall design: An equal volume of culture media was used to isolate EV and process to miRNA extraction according to manufacturer protocol (Qiagen)