Effect of D-glucose and L-glucose on gene expression of zebrafish embryos at 72 hpf (hours post fertilization) and 96 hpf.

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. Presently, we have established a zebrafish model that exhibits significant excessive angiogenesis induced by D-glucose and L-glucose. Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). We analyzed and compared the differentially expressed genes of control, high D-glucose-treated and high L-glucose-treated embryos. The results revealed that 1259 and 1074 genes were up-regulated significantly in high D-glucose and high L-glucose treated embryos, respectively, compared with control。 Embryos were treated with D-glucose and L-glucose at 24 hpf and then collected for transcriptome sequencing at 72 hpf and 96 hpf.