Effect of SOX9 dosage changes on gene expression in hESC-derived cranial neural crest cells [RNA-seq]

To assess the effects of quantitative SOX9 dosage changes on gene expression in hESC-derived cranial neural crest cells, we performed genome editing of the H9 hESC lines to tag SOX9 with FKBPV36, V5, and mNeonGreen. Upon differentiating edited hESC to cranial neural crest cells using an established protocol, addition of differing concentrations of the degrader molecule dTAGV-1 results in different SOX9 concentrations, as measured by flow cytometry. We then profiled gene expression profiling in each of these SOX9 concentrations Genome-wide gene expression profiling (RNA-Seq) of WT and SOX9-tagged H9 hESC-dervied cranial neural crest cells (CNCCs)