Colin K. Choi, Alan Rick Horwitz (2010) CIL:9530, Cricetulus griseus, epithelial cell. CIL. Dataset

Adhesion dynamics in a migrating CHO K1 cell. CHO K1 cells expressing paxillin-mEGFP were plated onto coverslips that were preincubated with a solution of 5 ug/ml fibronectin overnight at 4o C. The cells were visualized at 60x (1.45NA) with an Olympus IX70 inverted microscope using Total Internal Reflection Optics (through the objective mode) equipped with a Qimaging Retiga Exi CCD camera (0.1073 microns/pixel). Images were acquired every 5 seconds for a total of 15 minutes. Note the formation of small, nascent adhesions near the leading edge of protrusions. These adhesions form (in the lamellipodium) and their formation is coupled to actin polymerization. Many adhesions turnover (at the lamellipodium/lamellum interface), as the protrusion proceeds. Some adhesions also elongate and grow (mature), particularly during pauses in protrusion. The fraction of adhesions that elongate, and the rate at which they do so, is cell type dependent. Note also, the sliding and disassembly of adhesions in retracting regions of the cell. The movie was taken by Colin Choi in the Rick Horwitz laboratory (University of Virginia).

本数据集聚焦于迁移中的CHO K1细胞之粘附动力学。表达paxillin-mEGFP的CHO K1细胞被接种于预先用5ug/ml纤连蛋白溶液在4℃下过夜预孵育的载玻片上。利用配备Qimaging Retiga Exi CCD相机（像素尺寸为0.1073微米）的Olympus IX70倒置显微镜，在60倍（1.45NA）放大倍数下，采用总内反射光学（通过物镜模式）对细胞进行可视化。每隔5秒采集一次图像，共计15分钟。请注意，在突出部的前缘附近形成了微小的新生粘附结构。这些粘附结构在（波纹状伪足中）形成，其形成过程与肌动蛋白聚合相耦合。许多粘附结构（在波纹状伪足/伪足片层界面）发生周转，随着突出部的推进。一些粘附结构也会伸长并生长（成熟），尤其是在突出部暂停期间。伸长粘附结构的比例及其伸长速率取决于细胞类型。此外，还需注意细胞收缩区域中粘附结构的滑动和解聚。该视频由Colin Choi在Rick Horwitz实验室（弗吉尼亚大学）拍摄。