Spatial transcriptome analysis (Slide-seq2) of mouse main olfactory bulb

The goal of spatial transcriptome analysis is to profile the glomeruli position representing different olfactory receptors. 20 OB sections were obtained with equal spacing (130 um) along the anterior-posterior axis. In total, two sets of 20 slides were obtained from different animals. All of the Slide-seqV2 procedures were performed as previously described. Olfactory epithelial single cell suspensions made from 3 male and 3 female adult mice. Single-cell sequencing libraries were prepared using Chromium Single Cell 3' Reagent Kit V3 (10X Genomics) according to the manufacturer's guidelines. Libraries were sequenced using 150 cycles of paired end reads on Illumina Hiseq4000 and Novaseq6000 instruments (Novogene)空间转录组分析（spatial transcriptome analysis）的目标是解析表征不同嗅觉受体的嗅小球（olfactory glomeruli）的位置信息。研究人员沿前后轴以130 μm的等间距获取了20份嗅球（olfactory bulb, OB）组织切片。共计从不同实验动物体内获得了两组各20张切片玻片。所有Slide-seqV2实验流程均按照此前已发表的方案执行。研究人员从3只成年雄性与3只成年雌性小鼠的嗅觉上皮组织中制备了单细胞悬液。依照试剂盒厂商的操作指南，使用Chromium单细胞3'端试剂试剂盒V3（10X Genomics公司）构建单细胞测序文库。在Illumina Hiseq4000与Novaseq6000测序仪（诺禾致源公司）上，采用150个循环的双端测序模式对文库进行测序。