Excel tool with documentation for designing sgRNAs from the Casellas Lab, NIH. In addition, we have included the full protocol for making KOs in CH12 and mouse ES cells. Philippe found an error in pre
An integration reaction was performed with Cas1-Cas2 from Streptoccocus pyogenes into plasmids lacking and containing CRISPR loci. The resulting products were sequenced.
RNA-seq on HepG2 cells treated with a CRISPR gRNA against RPS25. (RPS25-BGHcLV20) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/R
