Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:25630, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 3 of Mouysset et al. Movie is CIL 25630 and original data file is CIL 35125.

本视频展示了通过RNA干扰技术耗竭cdc-48的秀丽线虫胚胎早期分裂直至四细胞期的过程。P0原虫体的首次秀丽线虫胚胎分裂是不对称的，生成一个前端的AB细胞以及一个较小的后端P1细胞。这两个细胞拥有不同的发育命运和分裂时间，AB细胞大约在P1细胞前2分钟进行分裂。时间间隔差分干涉对比显微镜（Time-lapse differential interference contrast, DIC）检测显示，cdc-48、ufd-1和npl-4的下调增加了P1细胞相对于AB细胞的细胞分裂延迟，从而导致延长了三细胞期。该视频对应于Mouysset等人在图1B和电影3中展示的实验。电影编号为CIL 25630，原始数据文件编号为CIL 35125。