Additional FISH images of methanotrophic (MOX, MITC849 probe double-labelled with 594 dye) symbionts in 10 μm sections of H. (S.) methanophila sponge (Mictlan Knoll individual), epifluorescence microscpopy
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https://figshare.com/articles/dataset/Additional_FISH_images_of_methanotrophic_MOX_MITC849_probe_double-labelled_with_594_dye_symbionts_in_10_m_sections_of_H_S_methanophila_sponge_Mictlan_Knoll_individual_epifluorescence_microscpopy/5161222
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资源简介：
We double-labelled MTC849 (5` – CGTTAGCTCCACCACTAAG – 3`) with Atto594 dye (Biomers, Ulm, Germany), and hybridized it with sponge tissue in 20% formamide buffer using standard protocol (Duperron <i>et al.</i>, 2008). Photomicrographs were acquired with Zeiss Axioplan 2 epifluorescence microscope (Zeiss, Jena, Germany), 40x or 100x lenses (see file name). Three channels were used - 594 (MITC849), DAPI and FITC(autofluoresence).
提供机构：
figshare
创建时间：
2018-06-10
