Promotion of Subtotal Uterine Reconstruction by a Tissue-engineered Uterus Biomimeticing Structure and Extracellular Matrix Microenvironment

To better characterize the ECM signature of the ECMEC-SIS and ECMSMC-PU/SIS, a 4D label free proteomic analyses was carried out. In total 2761 and 1520 proteins have been identified for the ECMEC-SIS and ECMSMC-PU/SIS, respectively, including 1055 in common . An ECM-specific categorization database, MatrisomeDB 2.0, was employed to analyze the ECM matrisome protein and ECM-associated proteins.In the ECMEC-SIS group, the ECM matisome, matrisome-associated, and other proteins have accounted for 17.87%, 17.00%, and 65.13%, respectively. However, the proportion of ECM proteins, especially glycoproteins and regulators, was greater in ECMSMC-PU/SIS group compared with the ECMEC-SIS group. In the ECMSMC-PU/SIS group, the ECM matisome, matrisome-associated, and other proteins have accounted for 26.39%, 20.85%, and 52.77%, respectively.