Transcriptomic profile of endogenous Tregs in mouse injured bone, muscle, skin and heart tissues compared to healthy spleen Tregs

We aimed to identify the changes in gene expression profile of Tregs isolated from the injured bone, muscle, skin and heart, compared to healthy (uninjured) spleen Tregs. The bone injury consisted of calvarial bone defects, the muscle injury involved volumetric muscle loss defect in the quadriceps and the skin injury involved full-thickness punch-biopsy wounds. The heart injury was performed using the left coronary artery ligation to induce myocardial infarction (MI). The Tregs accumulating within the injured tissues were isolated and purified by fluorescence activated cell sorting (FACS), at 7 days post-injury (which is the peak of Treg accumulation within these tissues post-injury). Overall design: Bone injury (cranial bone defect), muscle injury (quadriceps volumetric muscle loss), skin injury (full-thickness punch-biopsy wounds) or heart injury (myocardial infarction, MI by left coronary artery ligation) was induced in 10 week old male Foxp3(DTR/GFP) mice (1 injury per mouse) and the injured tissue was harvested 7 days after injury to sort Tregs by flow cytometry (n=3, each replicate containing cells pooled from 3 mice). As a control, healthy (uninjured) spleen Tregs from 10 week old male Foxp3(DTR/GFP) mice were also sorted by flow cytomtery (n=6). The sorted cells were then used for RNA extraction and bulk RNA sequencing. ***Please note that the Series annotations have been updated on July 25th, 2024, and additional sample records included on Aug 2, 2024**** ***Submitters state that the records will be updated with two additional samples (Bone injury replicate 2, and Bone injury replicate 3)****