Confirmation of fifth <i>Candida auris</i> clade by whole genome sequencing

<i>Candida auris</i> has emerged globally as a multidrug-resistant pathogen causing outbreaks in health care facilities. Whole genome sequencing (WGS) analysis has identified four major clades, while earlier WGS data from a single Iranian isolate suggested the existence of a potential fifth clade. Here, we confirm the existence of this fifth clade by providing WGS data of another four Iranian isolates. These clade V isolates differed less than 100 single-nucleotide polymorphisms (SNPs) between each other, while they were separated from the other clades by more than 200,000 SNPs. Two of these isolates were resistant to fluconazole and were found to harbour mutations in the <i>TAC1b</i> and <i>ERG11</i> genes.

耳念珠菌（Candida auris）已在全球范围内作为多重耐药病原菌出现，引发医疗机构内的暴发疫情。全基因组测序（WGS）分析已鉴定出四个主要进化枝（clade），而此前针对单株伊朗分离株的全基因组测序数据提示存在潜在的第五个进化枝。本研究通过提供4株伊朗分离株的全基因组测序数据，证实了该第五进化枝的存在。进化枝V的各分离株之间单核苷酸多态性（SNPs）差异不足100个，而它们与其他进化枝的SNP差异则超过20万个。其中2株分离株对氟康唑耐药，且携带TAC1b与ERG11基因的突变。

搜集汇总

背景与挑战

背景概述

该数据集通过全基因组测序（WGS）确认了Candida auris的第五个进化枝（clade V），基于四个伊朗分离株的数据，这些分离株之间的单核苷酸多态性（SNPs）差异小于100，而与其他进化枝的差异超过200,000 SNPs。数据集还揭示了部分分离株对氟康唑耐药，并携带TAC1b和ERG11基因突变，突出了其耐药性特征。