Transcriptome of Hirudo medicinalis in the response to different types of stress and stimulus. HMED_Stress_Transcriptome

Live individuals of Hirudo medicinalis from were subjected to several abiotic stress experiments: visible light, UVB stress, hyperoxia, hypoxia, and osmoregulation, plus control specimens. All samples were dissected into head, mid body and tail, flash frozen in liquid nitrogen and kept <-70ºC until further processing. RNA extractions after stress experiments were performed using the TRIzol reagent (Invitrogen, USA) method following the manufacturer’s instructions. Concentration of all samples was assessed by Qubit RNA BR Assay kit (Thermo Fisher Scientific). Samples were subjected to either Illumina’s TruSeq Stranded mRNA library preparation kit or Truseq stranded Total RNA Library with Ribo-Zero depending on the tissue and species, and sequenced on a NovaSeq 6000 (Illumina, 2 x 150 bp) for a 6Gb coverage.