The raw data of transcriptomes in whole brains when EcR-A was knocked down in pC1 neurons or not

The establishment of neural circuits for female sexual receptivity is
essential to reproduction, but the underlying mechanism of associated
neurodevelopment is still not fully understood. Here we found that
neuropeptide prothoracicotropic hormone (PTTH), hormone of the PG axis,
regulates virgin female receptivity through ecdysone in Drosophila
melanogaster. PTTH-positive PG neurons are doublesex-expressing neurons,
they regulate virgin female receptivity before the metamorphosis during
the 3rd-instar larval stage. Furthermore, the genetic knocking down of
ecdysone receptor EcR-A in pC1 neurons regulates the morphological
development of pC1 neurons and further the virgin female copulation rate.
However, the activity of pC1 neurons did not change, suggesting the
changes of synaptic connection between pC1 neurons and other neurons.
Analysis of brain transcriptomes when EcR-A was knocked down in pC1
neurons reveals that additional genes are regulated downstream of EcR-A in
pC1 neurons. Our work suggests a mechanism underlying how the
neurodevelopment of neural circuits regulates virgin female receptivity in
adults.