Mechanism of Sishen Pill on Ulcerative Colitis based on Network Pharmacology and animal experiments

Objective To explore the mechanism of action of Sishen Pill in the treatment of Ulcerative colitis (UC) through network pharmacology and animal experiments.Methods TCMSP and BATMAN-TCM databases were used to obtain the components and targets of Sishen Pill; GeneCards, OMIM, and PharmGKB databases obtain UC targets, and Cytoscape 3.8. 2 software builds the "Sishen Pill-Active Ingredient-Intersection Targets" interaction network and PPI network, and performs GO and KEGG enrichment analysis on the intersection targets, and then The core components are molecularly docked with their targets. A mouse model of UC with spleen and kidney yang deficiency was constructed, and Sishen Pill was given intervention treatment. The network pharmacological prediction was verified by WB and RT-qPCR experiments.Results The core components of Sishen Pill in the treatment of UC include quercetin, β-sitosterol, cholecalciferol, delphinium pigment, puerarin, etc. The core targets include IL-6, TP53, EGFR, AKT1, IL1β, etc., and mainly play a role through IL-17, PI3K-Akt, Toll-like receptor, TRP signaling pathway, etc. The core component has good binding performance to the core target; Sishen Pill can reduce the expression of EGFR and IL-6 and increase the expression of p53 in mouse colon tissue.Conclusion Sishen Pill can regulate EGFR, IL-6, p53 and other targets, regulate IL-17, PI3K-Akt, Toll-like receptors, and TRP signaling pathways, reduce inflammatory damage and treat UC.