Gene expression in day 4 pregnant uterus

To detect the gene profiles in receptive uterus, two independent uteri are collected from pregnant day 4 mice and subjected to RNA-Seq. After aligned to mouse mm9 by Tophat2, RPKM value was calculated by Edger. Our results show that HMGB1 is one of the most abundant gene in the whole HMG family. The expression of HMGB1 was further confirmed by qPCR, ISH and immunofluorescence. The expression of HMGB1 was found expressed in very cells, indicating the essential role of HMGB1 for reproduction. This RNA-Seq data provides fundamental information for our further physiological study of HMGB1. Total RNAs in whole uterus from two independent mouse were extracted by TRIzol and purified by ploy-A before subjected to RNA-Seq by HiSeq2500. The expression of level of each gene is normalized to RPKM.