Primer sequences and standard curve data for quantitative real-time PCR analysis.
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A: Regression coefficient of the standard curve;B: The slope of the standard curve;C: The amplification efficiency of the PCR. As detailed in introduction, MyoD, Myf5, MRF4 and myogenin are examples of MRFs. Akt and TSC1/2 are upstream targets of mTOR while 4EBP1, eIF4E, S6K and RPS6 are downstream targets. Pax7 and myostatin also control myoblast proliferation.
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2015-12-02



