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RNA sequencing analysis of wild-type and Scd1-deficient CD4+ naïve T cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE160040
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Analysis of differentially expressed genes in Scd1-deficient CD4+ naïve T cells. The goal of this study was to elucidate the underlying pathway of Scd1-deficiency leading to increased regulatory T cell differentiation. CD4+ T cells were purified from splenocytes (collected from 11-week-old wild-type and Scd1-deficient C57BL/6 mice) using magnetic separation. Subsequent fluorescence-activated cell sorted naïve T cells (CD4+ CD44med CD25- CD62L+) were lysed using QIAzol.
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2023-08-29
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