Primary and secondary transplantation of 10 highly purified JAK2V617F or wild type E-SLAM HSCs.

Donor bone marrow was harvested and E-SLAM HSCs isolated as shown in Figure 1E. Ten E-SLAM HSCs from JAK2V617F mice 6–10 mo following pIpC injection or their wild-type littermate controls were transplanted into each of five irradiated recipients alongside 200,000 competitor cells. The numbers of successful transplantations and their respective chimerism levels are shown above for both primary (10 E-SLAM HSCs) and secondary (6×106 bone marrow cells from the primary animal) transplantations. A successful transplantation was defined as those showing >1% donor peripheral blood chimerism (out of donor+competitor) at 16–24 wk and at least 0.5% peripheral blood chimerism within each lineage (GM, B, and T) at some point over the 16-wk period. For each primary mouse, two secondary transplantations were performed and the original parent is indicated. Mice showing trace amounts of repopulation (