Expression data of organoid derived from HSCs. Expression data of organoid derived from HSCs

To investigate the differences in mRNA expression profiles between TSG-6 or vehicle treated Hu-pHSCs and BM-MSCs organoid spheroids, we performed mRNA microarrays for total RNA extracts isolated from these spheroids (vehicle: CON/3D=3, TSG6: TSG-6/3D=4, BM: BM-MSCs3D=3) We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Overall design: For profiling, we established organoid spheroid as described previously hepatic organoid culture methods.