Fragaria x ananassa: FANhybrid_r1.2 (Strawberry whole-genome sequencing). Fragaria x ananassa strain:Reikou

The genome sequencing of cultivated strawberry (F. x ananassa) was performed in the two assembly methods taking into account the heterozygosity, because the F. x ananassa is octoploid species. One is the assembly named FANhybrid_r1.2, which was assembled to eliminate the heterozygous bases by integrating the homoeologous sub-genomes. The other is the assembly named FAN_r1.1, which was assembled with octoploidity. FANhybrid_r1.2: This assembly was performed by integrating the homoeologous sub-genomes to eliminate the heterozygous bases. First, the reads obtained by Roche 454 GS FLX+ were assembled by Newbler 2.7. Next, the reads obtained by Illumina GAIIx/HiSeq1000 were assembled by SOAPdenovo v1.05. The illumina scaffold sequences without similarity against the 454 scaffold sequences were further assembled by Newbler 2.7. After these assemblies, the scaffold sequences asssembled by Newbler 2.7 were merged.