scRNA-seq of stromal vascular fraction (SVF) of lipoma and bulk RNA-seq of lipoma-derived adipocytes.

We determined the gene signatures of adipocytes differentiated from lipoma SVF. Furthermore, single-cell RNA-sequencing was performed for SVF from inherited (familial) and sporadic lipoma. Patients undergoing surgical excision of lipomas were identified and the dermatologist clinically confirmed the diagnosis of lipoma. Tissues was enzymaticaly digested and SVF was obtained and diffrentiated in adipogenic media for 5-7 days before collection of the RNA.