Binding and transcriptional profile of Halobacterium salinarum histone

To understand the function of histones of halophilic archaea, genome-wide binding of the sole histone gene of Halobacterium salinarum, hpyA, was studied using ChIP-Seq studies with HA-tagged expressed on a plasmid using the native promoter. A strain with HA- tag-alone, also expressed from plasmid, was used as a negative control. This was studied in both exponential and stationary phases of growth, in both optimal media (4.2M NaCl) as well as reduced salt (3.4M NaCl). 4 biological replicates for each condition.Transcriptional effects of the gene were studied using RNA-Seq of parent and hpyA KO strains. All experiments were carried out in optimal media (4.2M NaCl) as well as reduced salt (3.4M NaCl) in exponential phase. 6 biological replicates for each condition.Knockout strains used in the study were verified by full-genome sequencing of gDNA (no replicates).