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Dataset of the second mass-spectrometry experiment to identify in vivo phosphorylation of CA-RACB.

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Figshare2022-03-25 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Dataset_of_the_second_mass-spectrometry_experiment_to_identify_i_in_vivo_i_phosphorylation_of_CA-RACB_/19422438
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Dataset comprising all recovered barley phosphopeptides from protoplasts of stable transgenic barley plants. Phosphopeptides were enriched from full-protein extracts of either HA- or HA-CA-RACB overexpressing protoplasts, which were co-transformed with GFP-RBK1 and incubated with MG132. Samples were decomplexed by fractionating phosphopeptides according to their hydrophobicity (S2B Fig). All peptides and their modifications were identified by mass spectrometry. Non-enriched full-protein samples (FP) were included in mass spectrometry analyses, but did not yield many phosphopeptides. Of main interest in this dataset are the gene identifiers and peptides with phosphorylated amino acids and their corresponding localization scores. (XLSX)
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2022-03-25
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