RNA-seq in the basal nodes of nitrogen- and phosphorus-stressed Triticum aestivum var Cadenza (wheat) plants.

The aim of this study was to examine the transcriptional changes that govern low nitrogen (N) and low phosphorus (P) responses in basal nodes and may be associated with tiller suppression under nutrient-limiting conditions. For this purpose, RNA-seq was conducted in 18 days-old hydroponically grown wheat plants 8 days after the introduction of the plants to the nutrient limitation. The three different treatments were: Control (10 mM N, 1 mM P), Low N (0.1 mM N, 1 mM P) and Low P (10 mM N, 0.01 mM P). In total, four biological replicates were included per treatment. RNA-seq was conducted in total RNA extracted from 6 basal node samples pooled together per biological replicate. In this study, the basal node is defined as the 0.5 cm of the main shoot base, which includes the apical meristem, lateral buds, leaf meristems etc.