System immunology characterization of novel vaccine formulations for Mycoplasma hyopneumoniae bacterins

In this study the immune response induced by six different mycoplasma hyopneumoniae bacterin vaccines after parenteral injection in pigs were characterized using transcriptomics based on RNA sequencing. The vaccines tested included the commercial vaccine Hyogen®, a PBS group as a negative control, cationic liposomes + C-di.AMP (Lipo_AMP), cationic liposomes + Toll-like receptor (TLR) 2/1, TLR7 and TLR9 ligands (TLR ligands; Lipo_TLR), microparticles + TLR ligands (PLGA_TLR), squalene-in-water emulsion + TLR ligands (SWE_TLR) or DDA:TDB liposomes (Lipo_DDA:TDB). Blood way collected at day 0, one day post vaccination (day 1) and 7 days post vaccination (day 7). RNA was sequenced from whole blood using Illumina-based RNA-seq.