ScRNA-seq Analysis of Whole Skin Cells from Irradiated and Naïve Mice

We report the transcriptomic analysis of single cells derived from irradiated and naive murine whole skin. The aim of the study was to elucidate the molecular and cellular drivers of irradiation-induced alopecia and dermatitis (IRIAD), two of the most visually recognized complications of radiotherapy treatments in human cancer patients. Overall design: Single-cell RNA-seq analysis was performed on pooled, viable cells isolated from whole skin samples derived from either irradiated (15 Gy) female (C57BL/6) mice 14 days post-treatment (n=4), or from age-matched naïve (C57BL/6) controls (n=4).