Distinct patterns of somatic alterations between normal and tumor genomes

The main objective of this project is to develop a sequencing strategy to efficiently define the complete catalog of mutations present in the breast tumor cell line HCC1954. Strategies for targeting gene rich regions of the genome, such as exon capture and construction of reduced representation libraries will be combined with shotgun and paired-end sequencing. Parallel sequencing of a lymphoblastoid cell line (HCC1954BL), derived from the same breast cancer patient, will be use to accurately distinguish mutations from polymorphisms.