Small RNA sequencing from transgenic Arabidopsis 35S:GUS (lines 6b4 and L1) and 35S:SUG (line SUG6)
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下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP234703
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资源简介:
Transgene loci were transfered in different Arabidopsis thaliana mutant backgrounds by crossing. Small RNA molecules were extracted and sequenced to survey the influence of mutations on transgene small RNA biogenesis. Overall design: Whole plants were grown in vitro, harvested 11 days after germination and grinded to extract total RNA with phenol/chloroform and ethanol precipitated. RNA samples were sent to GATC Biotech for size-selection, cloning and sequencing on HiSeq2000 platform.
创建时间:
2019-12-07



