Supporting data for Thesis - “Characterisation of Double-Negative B and Plasma B Cells in the Tumour Microenvironment of NPC”

Name: Supporting data for Thesis - “Characterisation of Double-Negative B and Plasma B Cells in the Tumour Microenvironment of NPC”
Creator: HKU Data Repository
Published: 2025-09-30 03:30:56
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DataCite Commons2025-09-30 更新2026-05-03 收录

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https://datahub.hku.hk/articles/dataset/Supporting_data_for_Thesis_-_Characterisation_of_Double-Negative_B_and_Plasma_B_Cells_in_the_Tumour_Microenvironment_of_NPC_/30009121

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资源简介：

This dataset contains raw and processed data generated for the doctoral thesis, “Characterisation of Double-Negative B and Plasma B Cells in the Tumour Microenvironment of NPC”. It includes.RDS files containing single-cell RNA-sequencing (scRNA-seq) dataset derived from NPC tumour samples, NPC patient-derived blood samples, non-malignant tissue samples and nasopharyngeal organoid; as well as those from a scRNA-seq dataset derived from co-culture experiments where naive B cells were co-cultured with EBV-positive (EBV+) and EBV-negative (EBV-) NPC43 cells. Excel files of processed single-cell BCR-sequencing (scBCR-seq) data used in the study. Excel files of raw data of ELISA assays to detect MIF in EBV+ NPC and EBV- NPC cell culture supernatant, as well as flow cytometry gating of B cell subsets in coculturing experiments, including Naive B cells with EBV+ vs EBV- NPC, with the stimulation of BAFF + anti-IgM antibodies; Naive B cells with EBV+ NPC, under treatment of 3.75 and 7.5 ug/ml of anti-IgG or anti-TNFRSF14 antibodies, with the stimulation of BAFF + anti-IgM antibodies; Naive B cells cultured with 50 ng/ml and 100 ng/ml MIF or without MIF, with the stimulation of BAFF + anti-IgM antibodies; and Naive B cells with normoxia versus hypoxia EBV+ NPC, with the stimulation of BAFF + anti-IgM antibodies. Excel files of analysed/processed data of H2DCFDA and MTT assay between normoxic versus hypoxic EBV+ NPC43 cells. Excel files of patient data within scRNA-seq data and tonsil sample collection.This dataset comprises RDS files and Excel files representing both bioinformatic and experimental data, and supports the reproducibility of the findings described in the thesis.

提供机构：

HKU Data Repository

创建时间：

2025-08-29