Identifying ChCPC1-dependent and ChATF1-dependent changes in gene expression of C. heterostrophus

To delve into the roles of ChCPC1 and ChATF1 in C. heterostrophus, we compared the transcriptome of Chcpc1 or Chatf1 with that of WT by RNA-seq with three biological replicates. Feature Counts v.1.5.0-p3 was used to count the reads numbers mapped to each gene. Then, the fragments per kilobase million (FPKM) of each gene were calculated based on the length of the gene and the number of reads mapped to the respective gene.