Table_5_Establishment of stable expression of firefly luciferase and EGFP in a canine inflammatory mammary carcinoma cell line and tumor-bearing model in nude mice.XLSX

Canine inflammatory mammary carcinoma (CIMC) is a type of canine malignant mammary tumor with a poor prognosis and high mortality. We transduced firefly luciferase and enhanced green fluorescent protein (EGFP) into CHMp, a CIMC cell line, and established CHMp-Luc-EGFP cells. We investigated the characteristics of this cell line in vitro and in vivo. CHMp-Luc-EGFP was passaged continuously 75 times, with stable expression of luciferase and EGFP. Compared with the wild-type, CHMp-Luc-EGFP had similar proliferation, metastasis, histopathology characteristics, and expression of E-cadherin, N-cadherin, and Ki-67. A tumor-bearing model was established by implantation of CHMp-Luc-EGFP cells, and the dynamic changes of tumors were visualized and quantified using the IVIS imaging system. In summary, the cell line we established could reflect the biological characteristics of CHMp cells, visualize the tumor progression in vivo, and provide a powerful tool for the study of CIMC.

犬类炎症性乳腺癌（CIMC）是一种预后不良、死亡率高的犬类恶性乳腺肿瘤。本研究将萤火虫荧光素酶和增强型绿色荧光蛋白（EGFP）转染至犬类炎症性乳腺癌细胞系CHMp中，构建了CHMp-Luc-EGFP细胞。我们对该细胞系在体外和体内的特性进行了研究。经过连续传代75次，CHMp-Luc-EGFP细胞中荧光素酶和EGFP的表达保持稳定。与野生型细胞相比，CHMp-Luc-EGFP细胞在增殖、转移、组织病理学特征以及E-钙粘蛋白、N-钙粘蛋白和Ki-67的表达方面表现出相似性。通过植入CHMp-Luc-EGFP细胞建立了肿瘤模型，利用IVIS成像系统对肿瘤的动态变化进行了可视化和量化。总之，我们建立的细胞系能够反映CHMp细胞的生物学特性，可视化体内肿瘤的进展，并为犬类炎症性乳腺癌（CIMC）的研究提供了强大的工具。