Lyplal1_knockout_mouse_RNAseq

Several GWAS studies have found an association between variants close to the Lysophospholipase-like 1 (Lyplal1) locus and metabolic traits, including central obesity, fatty liver and waist-hip ratio (where the effect is more significant in females than males). Lyplal1 is also upregulated in the adipose tissue of obese patients, and a mutation has been identified in a lipodystrophic patient with no other known mutation (David Savage, personal communication). However, the physiological role of Lyplal1 is not fully understood. We investigated the role of Lyplal1 using a knockout mouse model. Lyplal1 knockout mice (from the KOMP repository) were placed on a high fat diet (HFD) at 6 weeks of age. RNAseq analysis is being performed on samples from liver, gastrocnemius muscle and two adipose depots (visceral and subcutaneous) collected from 28 week old mice, to investigate the expression changes caused by knockout of Lyplal1, and how this differs between the tissues.