Pan-AcH3 ChIP-chip with Custom High Resolution Tiling Arrays on HUVECs and HuAoVSMCs

Differential EC enrichment of pan-AcH3 was assessed at 34 select genes, including 19 EC-enriched genes, 6 broadly expressed genes, and 9 EC-excluded genes, by pan-AcH3 (AcH3K9 and AcH3K14) ChIP-chip analysis on human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HuAoVSMCs) with a high resolution tiling DNA microarray. Specifically, the tiling array analyzes the 50 kb genomic region upstream of transcription initiation, the intragenic regions, and 50 kb genomic region downstream of the last exon. Pan-AcH3 ChIP was conducted on HUVECs and HuAoVSMCs. These ChIP samples were hybridized to a custom 1 M agilent microarray for ChIP-chip analysis.