Description of bulk RNA-seq samples.

Shown are the 966 bulk RNA-seq samples used in this study, including: source (iPSCORE or GTEx); subject ID; assay ID; SRA run ID for the GTEx RNA-seq samples downloaded from dbGaP; iPSCORE iPSC line identifier submitted to dbGaP (phs001325), iPSCORE unique differentiation identifier (UDID) assigned to all molecular data generated from same iPSC-CVPC differentiation; total number of reads; % uniquely mapped reads; % of mitochondrial reads, calculated as the number of reads mapping to mitochondrial genes divided by the total number of reads mapping to genes; tissue and organ associated with each sample (arteria, heart, iPSC-CVPC, aorta, coronary artery, atrial appendage or left ventricle: 0 = absent; 1 = present); 50 principal components calculated on the expression of 2,000 genes across the 966 CVS samples using Seurat; UMAP coordinates of each sample after clustering by Seurat; S phase and G2M and scores calculated using Seurat; pseudotime score of each sample using Monocle; cluster membership of each sample calculated using Seurat at four different resolutions (Figs 1A and 1B, S2 and S3); estimated cell type proportions deconvoluted using CIBERSORT; the last column (“Trimmed”) indicates which iPSCORE samples had their read length trimmed to 75 bp to test whether different read lengths between iPSCORE and GTEx affect differential gene and isoform expression analyses.
(CSV)