Yeast double strand break resection sequencing

This project applied targeted next generation sequencing to an ILV1 allele in yeast that had been subjected to a site-specific DNA double-strand break, in parallel with an unbroken control allele. The method included primer extension and molecule circularization with unique molecular identifiers. After aligning reads to the two alleles, normalized DSB signal was used to characterize resection over time for wild-type and a series of mutant yeasts including mre11, exo1, sgs1 and others.