Comparative analysis of whole genome sequence of Leishmania donovani strain 1S (wild type), Leishmania donovani strain 1S (set7-null mutant), and Leishmania donovani strain 1S (set29 mutants).

This study was performed with the goal of understanding the roles of Leishmania donovani SET7 and SET29 proteins. Using homologous recombination to knock out set7 and set29 genes, set7-null (set7-/-) and set29 (set 29-/+ and set29-/-/+) mutant parasites were created. This was followed by whole genome sequence analyses of wild type, set7-/-, set 29-/+ and set29-/-/+ parasites. Our data reveals, that in wild type parasites almost all the chromosomes are disomic except chromosome 26 which is trisomic and chromosome 31 which is tetrasomic. The set7-null mutant exhibited somy changes of 5 chromosomes (2, 8, 11, 26, and 29). The two set 29 mutants exhibited somy changes of 2 chromosomes.