Transcriptome of Escherichia coli O157:H7 (Sakai) in response to spinach leaf lysates by RNA-seq

E. coli O157:H7 can use vegetables such as spinach as secondary hosts, from where they can be transmitted into the food chain. Our previous microarray analysis showed whole-scale changes in gene expression of E. coli driven in a large part in response to plant metabolites. The aim of this work was to expand beyond the inherent limitations of microarray analysis, taking an RNA-seq approach to analyse the same samples.