A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission

Animals generate a wide range of highly coordinated motor outputs, which allows them to execute purposeful behaviors. Individual neurons in the circuits that generate behaviors have a remarkable capacity for flexibility, as they exhibit multiple axonal projections, transmitter systems, and modes of neural activity. How these multi-functional properties of neurons enable the generation of adaptive behaviors remains unknown. Here we show that the HSN neuron in C. elegans evokes multiple motor programs over different timescales to enable a suite of behavioral changes during egg-laying. Using HSN activity perturbations and in vivo calcium imaging, we show that HSN acutely increases egg-laying and locomotion while also biasing the animals towards low-speed dwelling behavior over minutes. The acute effects of HSN on egg-laying and high-speed locomotion are mediated by separate sets of HSN transmitters and different HSN axonal projections. The long-lasting effects on dwelling are mediated by H..., GCaMP datasets were collected from freely-moving C. elegans animals, using widefield imaging of animals on agar slides, as described in the manuscript., ImageJ tracking script:  Put the .txt file and the .ijm file in the same folder under the \"macros\" folder in ImageJ/Fiji installation. The .txt file includes settings that can be adjusted for the neuron of interest. the .ijm file is an ImageJ macro
Neuron retracking script: MATLAB code that enables tracking of fluorescence intensity in the same neuron location in different channel. The inputs required are a txt file containing the neuron location (which is the output of the ImageJ tracking script), and Tiff files from the recordings.
For HSN GCaMP dataset: there are 6 file folders: 1) gcamp raw 2) mscarlet raw 3) Ratiometric are in .mat files (MATLAB) ; 4) Eggevetns 5) GcampPeaks and 6) speed are in txt file format 
For BAG GCaMP dataset: each folder contains a JPEG file to define fused agar boundary and a gcamp tracking file (a csv with: frame,xcoord,ycoored,intdens,intsub,bgmedian,maxint,area,x,y,sqintdens,sqintsub,sqarea,threshold,animal,redFlag,useTracking), ##A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission

This dataset is from the study “A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission” by YC Huang, J Luo, W Huang, CM Baker, MA Gomes, AB Byrne, and SW Flavell (2023).
GCaMP datasets were collected from freely-moving animals placed on agar with seeded OP50 bacterial food. The HSN neuron calcium imaging aimed to investigate the activity of the HSN neuron related to the animals' locomotion and egg-laying behavior on food. The BAG neuron calcium imaging aimed to observe whether the BAG neuron responds to the osmotic upshift.

## Description of the data and file structure

The HSN\_GCaMP\_data folder contains six subfolders that provide HSN activity and animals' behaviors from indicated recordings, labeled with the date of recording in the file name.

1. The EggEvents folder contains .txt files that show the frame(s) in which an anim...