Comparative transcriptomic profiling of overexpression of PsrA in Pseudomonas aeruginosa PAO1

PsrA, a transcription factor belonging to the TetR family, is known to participate in the regulation of fatty acid metabolism, type III secretion system, and quinolone signaling in Pseudomonas aeruginosa. Using a psrA overexpression strain, this study conducted a transcriptomic analysis to examine the role of PsrA in P. aeruginosa PAO1. We cloned psrA in the multicopy plasmid pMMB66EH and then introduced the recombinant plasmid into P. aeruginosa PAO1. The psrA overexpressing strain, named PAO1 [pMMBpsrA], was examined and compared with the parental strain PAO1 carried the empty vector pMMB66EH, named PAO1[pMMB66EH].